Expulsión de espermatozoides por acción hipofisaria en el sapo

Abstract

En el Bufo arenarum Hensel la espermatogénesis se produce en espermatocistos o folículos, esto es en generaciones localizadas que provienen de una espermatogonia primaria única. Esta se reconoce por su gran tamaño y su núcleo polilobuladó y está rodeada por células foliculares endoteliformes que le forman una envoltura completa. La espermatogonia primaria se divide repetidamente y forma un espermatocisto esférico de espermatogonias secundarias;

The injection of pars distalis produces the liberation of spermatozoa in toads testicles. The spermatozoa then pass through the kidney and appear in the urine. This action is also produced by chorionic gonadotrophin, even in hypophysectomized toads, by that of the serum of pregnant mares (confirming Galli Mainini) by the luteo stimulating hormone (ICSH) and to a less extent by the folli- ele stimulating hormone (FSH) of the pituitary of sheep, rat, man and the frog Leptodactylus ocellatus (L.) Gir. The pituitaries of several animals were inactive and also other organs of the toad, and several other substances. By intratesticular injection a local action is obtained in the injected testicle. The reaction occurs at 23°C and is slower at 4°C. It takes place in the absence of the nervous system, liver, lungs, pancreas or pituitary. Gonadotrophins can be determined semi- quantitatively. Lesions of the tuber can produce pituitary necrosis with a reabsorption of gonadotrophin and elimination of spermatozoa.